Exer Discussion

The different pigments showed an increasing absorbance from wavelengths 400-450 nm (violet-blue) and from 600-650 nm (orange-red) while their absorbance from wavelengths 450-600 nm (wavelength of green) are very low. Although they have a similar trend, their values are not the same. The dark green pigment has the highest absorbance in all the wavelengths relative to the other pigments and the yellow pigment has the lowest absorbance. Each pigment has a unique absorption spectrum because they absorb photons that correspond to the energy difference between their ground and excited state.

Wavelengths around 400-500 nm and 600-650 nm should be used to detect absorption thus acknowledging the presence of the pigments. If you use the wavelength for green light, you will just obtain a very low value because around this wavelength, light is transmitted.

Increasing the concentration of a solution would mean increasing the molecules of that solution that is capable of absorption of certain wavelengths. Therefore, the higher the concentration of the solution, the higher the light absorption at certain wavelengths.

Spectrophotometry can be used in determining the concentrations of different chemical solutions for they absorb light at different levels. It is also used in DNA and RNA analysis for nucleic acids naturally absorbs UV light.